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. 2020 Sep 8;12(9):2733. doi: 10.3390/nu12092733

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Impact of storage conditions on the whole peptidome. (A) Coefficient of variance (CV) in individual breast milk samples A–D due to storage at different conditions in comparison to technical (techn.) and biological (biol.) CVs. Lines indicate median values. Whiskers indicate 1–99 percentile to display outliers. (B) Principal component analysis of all peptides per individual sample reveals (i) high variance between the peptide pattern in breast milk from different participants, and (ii) highest effect on the peptide pattern after storage at RT for 24 h.