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. 2020 Sep 8;12(9):2733. doi: 10.3390/nu12092733

Figure 5.

Figure 5

Behavior of breast milk peptides depending on storage conditions in comparison to immediate freezing of samples at −80 °C. (A) Histograms of peptide ratios (cond 2–5 vs. cond 1, −80 °C) exemplarily shown for sample A. Ratios close to 1 display minor alteration during storage. High and low ratios point to strong impact of storage temperature. The accumulation of peptides with ratios of approx. 0.25 after storage of samples for 24 h at RT point to degradation of peptides. (B) Stability of 255 peptides randomly chosen from 1575 peptides quantified in total in sample A. The majority of peptides kept quite stable when stored at −20 °C or at 4 °C, but showed strong alterations when stored at RT. (C) Stability of selected peptides with the same pattern in samples A–D. The pattern displays events pointing to degradation, but also peptides with increasing levels were detected. Strongly decreasing at RT: top: QPSTQIVANAKGAVT (perilipin-2); middle: IPASSLPRLTPWIVA (butyrophilin subfamily1 member A1); strongly increasing at RT: bottom: VLPIPQQVVPYPQRAVPVQA (ß-casein).