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. 2020 Sep 16;12(9):1032. doi: 10.3390/v12091032

Figure 1.

Figure 1

Single versus multiple transient recruitments of ALIX into HIV Gag VLPs versus HIV Gag (YP−) VLPs. HeLa cells stably expressing ALIX–h30–eGFP were transfected with 1500 ng of Gag–mCherry (A,B) or Gag (YP−)–mCherry (C,D) and imaged 4 h after transfection. Assemblies of individual representative VLPs are shown with intensity plots (as gray dots and fitted with gray line) and cropped TIRF images of the Gag (top, gray) and ALIX (bottom, Blue) for (A) Gag-mChery VLPs and (C) Gag (YP−)–mCherry VLPs. Histograms of the first time (dark blue) and later recruitments (light blue) of ALIX are shown for (B) Gag-mChery VLPs and (C) Gag (YP−)–mCherry VLPs. The majority of the first ALIX recruitment took place within 1–10 min after the VLP assembly completing during both YP− and WT assembly.