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. 2020 Sep 25;44(5):2185–2197. doi: 10.3892/or.2020.7779

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — LINC01554 overexpression suppresses migration, invasion, and EMT-related gene expression in HCC cells. (A) Wound-healing assay in LINC01554-overexpressing SK-Hep1 and HCCLM9 cells. (B) Invasion ability of LINC01554-overexpressing SK-Hep1 and HCCLM9 cells. (C) Expression levels of EMT markers (ZO-1, E-cadherin, N-cadherin and vimentin) in LINC01554-overexpressing HCCLM9 and SK-Hep1 cells are analysed by western blotting. (D) FISH analysis of LINC01554 in the SK-Hep1 and HCCLM9 cells. The U6 and 18S rRNA probes are labelled red, the LINC01554 RNA probe is labelled green, and nuclear DNA with DAPI staining is labelled blue. Scale bar, 50 µm. All results are from at least three independent experiments. *P<0.05, **P<0.01, comparison with the Lv-NC group. HCC, hepatocellular carcinoma; EMT, epithelial-mesenchymal transition.