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. 2020 Aug 20;12(9):912. doi: 10.3390/v12090912

Figure 1.

Figure 1

Identification of pseudorabies virus (PRV) gE and gB proteins from the pMAL-c5x plasmid via SDS-PAGE. (A) M, molecular weight marker; lane 1, untreated E. coli pMAL-c5x; lane 2, E. coli pMAL-c5x after induction by IPTG; lane 3, untreated E. coli pMAL-c5x PRV gE; lane 4, precipitate of E. coli pMAL-c5x PRV gE after induction by IPTG and ultrasonic disruption; lane 5, supernatant of E. coli pMAL-c5x PRV gE after induction by IPTG and ultrasonic disruption. (B) M, molecular weight marker; lane 1, untreated E. coli pMAL-c5x; lane 2, E. coli pMAL-c5x after induction by IPTG; lane 3, untreated E. coli pMAL-c5x PRV gB; lane 4, precipitate of E. coli pMAL-c5x PRV gB after induction by IPTG; lane 5, supernatant of E. coli pMAL-c5x PRV gB after induction by IPTG and ultrasonic disruption; lane 6, precipitate of E. coli pMAL-c5x PRV gB after induction by IPTG and ultrasonic disruption.