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. 2020 Aug 20;12(9):912. doi: 10.3390/v12090912

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Statistical analysis of PRV gB and gE ELISA results. Reactivity of the PRV gB sera according to (A) the ROC curve analysis with (B) the criterion value (OD > 0.399 is positive and OD < 0.399 is negative) of the assay (0.399) denoted by *. Reactivity of the PRV gE sera according to (C) the ROC curve analysis with (D) the criterion value of the assay (0.324) denoted by *. The results showed that the cut-off value of gB ELISA was 0.399, the sensitivity was 95.83%, and the specificity was 91.37% (Figure 10B); the critical value of gE ELISA was 0.3191, the sensitivity was 91.7%, and the specificity was 88.89% (Figure 10D). We used SigmaPlot 10.0 for the analysis of the sensitivity and specificity of the assay. +LR is positive likelihood ratio; −LR is negative likelihood ratio.