Table 1.
Retention time (tR), asymmetry factor (As), and theoretical plate number per meter (N/m) values obtained for cytisine and N-methylcytisine on various columns with different mobile phases.
| Column | Mobile Phase | Cytisine | N-methylcytisine | ||||
|---|---|---|---|---|---|---|---|
| tR | As | N/m | tR | As | N/m | ||
| Hydro-RP | 5% MeCN + 20% acetate buffer at pH 3.5 H2O + 0.025 ML−1 DEA | 1.69 | 5.87 | 9860 | 4.69 | 3.78 | 16,970 |
| 5% MeCN + H2O + 0.025 ML−1 NaBF4 | 10.10 | 0.82 | 45,000 | 10.36 | 0.84 | 39,040 | |
| 5% MeCN + H2O + 0.025 ML−1 NaPF6 | 2.67 | * | 3.05 | * | |||
| 5% MeCN + H2O + 0.025 ML−1 IL BF4 | 3.41 | * | 3.38 | * | |||
| 5% MeCN + H2O + 0.025 ML−1 IL PF6 | 4.36 | * | 11.23 | 0.66 | 3500 | ||
| Phenyl-Hexyl | 5% MeOH + 20% acetate buffer at pH 3.5 H2O + 0.025 ML−1 DEA | 2.64 | 0.96 | 14,120 | 2.75 | 1.10 | 20,620 |
| 5% MeCN + H2O + 0.025 ML−1 NaBF4 | 3.44 | * | 3.44 | 0.65 | 18,090 | ||
| 5% MeCN + H2O + 0.025 ML−1 NaPF6 | 9.56 | * | 11.14 | * | |||
| 5% MeCN + H2O + 0.025 ML−1 IL BF4 | 2.53 | * | 2.72 | 1.48 | 1720 | ||
| 5% MeCN + H2O + 0.025 ML−1 IL PF6 | 3.75 | * | 5.83 | * | |||
| Polar RP | 5% MeCN + 20% acetate buffer at pH 3.5 H2O + 0.025 ML−1 DEA | 3.49 | 0.63 | 15,430 | 4.32 | 2.02 | 20,130 |
| 5% MeCN + H2O + 0.025ML−1 NaBF4 | 4.85 | * | 5.46 | * | |||
| 5% MeCN + H2O + 0.025 ML−1 NaPF6 | 9.93 | 0.73 | 27,270 | 12.46 | 0.79 | 23,300 | |
| 5% MeCN + H2O + 0.025 ML−1 IL BF4 | 5.99 | * | 6.91 | * | |||
| 5% MeCN + H2O + 0.025 ML−1 IL PF6 | 4.88 | 1.16 | 9960 | 9.22 | 1.18 | 9070 | |
| HILIC A | 90% MeCN + formic buffer at pH 4.0 | 4.08 | 1.09 | 39,570 | 3.03 | 1.39 | 37,620 |
| HILIC B | 90% MeCN + formic buffer at pH 4.0 | 2.58 | * | 2.09 | * | ||
| HILIC N | 90% MeCN + formic buffer at pH 4.0 | 8.00 | 1.10 | 4920 | 3.31 | 1.48 | 20,600 |
| SCX | 25% MeCN + formic buffer at pH 4.0 | 12.43 | 1.15 | 55,000 | 17.58 | 1.38 | 46,200 |
* fuzzy peak.