Table 5.
List of tested stationary phases and their physicochemical properties.
| Phase | Functional Group | Length (mm) | I.D. (mm) | Endcapped | Particle Size (μm) | Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | Recommended pH Range |
|---|---|---|---|---|---|---|---|---|---|
| Synergy Polar RP | Ether-linked phenyl | 150 | 4.6 | Proprietary (polar group) | 4 | 80 | 475 | 11 | 1.5–7.0 |
| CSH Phenyl-Hexyl | Phenyl-hexyl | 150 | 4.6 | Proprietary | 5 | 130 | 185 | 15 | 1.0−11.0 |
| Synergi Hydro-RP | Octadecyl (C18) | 150 | 4.6 | Proprietary (polar group) | 4 | 80 | 475 | 19 | 1.5–7.5 |
| ACE HILIC-A | Proprietary SIL | 150 | 4.6 | NO | 5 | 100 | 300 | − | 2.0–7.0 |
| ACE HILIC-B | Proprietary Aminopropyl | 150 | 4.6 | NO | 5 | 100 | 300 | 4 | 2.0–7.0 |
| ACE HILIC-C | Proprietary Polyhydroxy | 150 | 4.6 | NO | 5 | 100 | 300 | 7 | 2.0–7.0 |
| Luna SCX | Benzene Sulfonic Acid | 150 | 4.6 | NO | 5 | 100 | 400 | 0.55 Sulfur Load |
2.0–7.0 |