Figure 1.

Contig map of Apostichopus californicus flavivirus-like contig 91. The open reading frame matched a flavivirus polyprotein by BLASTx [37]. Methyltransferase, NS5 and helicase domains were identified by comparison against the conserved domain database (CDD) at NCBI [41]. The location of the envelope region was determined by protein folding comparison in Phyre [39]. The hairpin like structure preceding the Envelope region was determined by folding all sites between start (AUG) codons by mFold [40].