Figure 7.

Prevention of apoptosis-induced cell death: Vero cells (24-well plate format, 2.5 × 10⁵ cells/well, triplicates) were infected (MOI 0.1) with Paraiba/2015. After 2 h viral absorption, infected cells were treated with the indicated concentrations of compounds (0, 0.1, 1, and 10 EC₅₀) (A) Antimycin A, (B) OSU-03012 and Obatoclax, (C) Azaribine, Azauridine, and Pyrazofurin, (D) Mycophenolate mofetil, Mycophenolic acid, and AVN-944, (E) Brequinar, and (F) Aurintricarboxylic acid (ATA); and Caspase 3/7 levels were measured at 24, 48, and 72 h post-treatment. Data of each time point was compared to mock-infected control cells and expressed as mean of relative percentage and SD from three independent experiments conducted in triplicates. Statistical analysis was conducted by an unpaired Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, or no significance (n.s.).