Table 2.
Characterization of substrate binding pocket amino acid residues adenylation domains of NcpA and NcpB modules based on gramicidin S synthetase (GrsA) phenylalanine activating domain. Residues in brackets mark inconsistency with AY167420.1 residues.
| NRPS Module | Adenylation Domain Residue Position | Proposed Substrate | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 235 | 236 | 239 | 278 | 299 | 301 | 322 | 330 | 331 | ||
| NcpA1 | D | A | S | T | [I] | A | A | V | C | Tyr |
| NcpA2 | D | I | L | Q | L | G | L | I | W | Gly |
| NcpA3 | D | A | W | Q | F | G | L | I | D | Gln |
| NcpB1 | D | A | F | F | L | G | V | T | F | Ile/Val |
| NcpB2 | D | V | W | H | I | S | L | I | D | Ser |
| NcpB3 | D | V | Q | [F] | I | A | H | V | A | Pro/MePro |
| NcpB4 | D | A | W | [T] | I | G | [A] | V | C | Phe/Tyr/Leu |