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. 2020 Sep 17;18(9):470. doi: 10.3390/md18090470

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of IPA and IOE on the cell viability and melanin content of B16F10 melanoma cells. Cytotoxicity of IPA (A), IOE (B) in B16F10 melanoma cells. Cells were incubated with different concentrations of IPA, IOE for 72 h and cell viability was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Results are normalized to control. After 72-h incubation, absorbance was measured at 450 nm. Melanin content of group of IPA (C), group of IOE (D) in B16F10 cells. Melanin contents are expressed as percent values. The data are presented as means ± SD of independent experiments; ns, not significant; ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to no sample treated group.