Figure 4.

Effect of mutations on CDT-induced DNA damage. Representative images of γH2AX immunofluorescence and associated γH2AX quantifications from HeLa cells treated with 35 ng/mL of HducCDT mutants for 24 h. Quantifications of γH2AX signal are represented as the mean fluorescence intensity per cell (normalized to 1 for the untreated condition) or as the proportion of γH2AX-positive cells. Results present the mean ± SD of at least three independent experiments. Statistical differences were analyzed between each CDT treatment and the untreated condition (****, p-value < 0.0001) and between CDT mutants and the WT CDT (##, 0.001< p-value < 0.01; ####, p-value < 0.0001). NT: Untreated cells. Scale bar: 50 µm.