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. 2020 Aug 19;12(9):530. doi: 10.3390/toxins12090530

Figure 6.

Figure 6

Correlation between CdtB intracellular localization and DNA damage induction in cells expressing CdtB. Representative images of γH2AX immunofluorescence, mCherry fluorescence, and associated mCherry signal quantifications from HeLa cells transfected with pmCherry-C1-HducCdtB mutants with or without chromatibody (Cb) for 14 h. Quantifications are represented as the ratio for each individual cell of the nuclear over cytoplasmic mCherry signal. Results present every cell analyzed over three independent experiments and the mean ± SD of these cells. Statistical differences were analyzed between CDT mutants and the WT CDT (##, 0.001 < p-value < 0.01; ###, 0.0001 < p-value < 0.001; ####, p-value < 0.0001) and between the chromatibody-fused mutants and the unfused corresponding mutants ($$$$, p-value < 0.0001). NT: Untreated cells. Scale bar: 50 µm.