. 2020 Aug 18;3(5):883–895. doi: 10.1021/acsptsci.0c00054

Table 3. Summary of Photoswitchable NAM Dissociation Kinetic Parameters Estimates under Different Light Conditions as Determined by MS Binding Assays^a.

	Dark		500 nm		380 nm
Allosteric ligand	k_off (min^–1)^b	t_1/2 (min)^c	k_off (min^–1)^b	t_1/2 (min)^c	k_off (min^–1)^b	t_1/2 (min)^c
Alloswitch-1	0.14 ± 0.01	5.04 ± 0.10	0.25 ± 0.01*	2.77 ± 0.14	0.93 ± 0.16****	0.81 ± 0.15
MCS0331	0.07 ± 0.01	10.70 ± 0.95	0.11 ± 0.01*	6.32 ± 0.29	0.47 ± 0.07****	1.61 ± 0.28

Difference in the dissociation rate was determined by one-way ANOVA, which is performed on the logarithm of the k_off values. *, P < 0.1, and ****, P < 0.0001 vs dark condition for 500 and 380 nm conditions, respectively.

Dissociation rate of the indicated NAM.

t_1/2 is the half-life of dissociation.

Table 3. Summary of Photoswitchable NAM Dissociation Kinetic Parameters Estimates under Different Light Conditions as Determined by MS Binding Assaysa.

Table 3. Summary of Photoswitchable NAM Dissociation Kinetic Parameters Estimates under Different Light Conditions as Determined by MS Binding Assays^a.