Table 3.
Offspring lipogenic gene expression in male and female offspring at 4 weeks of age.
| Experimental Group | Male | Female | ||||||
|---|---|---|---|---|---|---|---|---|
| High LA (18% Fat) |
High LA (36% Fat) |
Low LA (18% Fat) |
Low LA (36% Fat) |
High LA (18% Fat) |
High LA (36% Fat) |
Low LA (18% Fat) |
Low LA (36% Fat) |
|
| 4 Week Offspring | ||||||||
| Liver | ||||||||
| Fasn | 0.91 ± 0.12 | 0.93 ± 0.15 | 1.49 ± 0.42 | 0.66 ± 0.08 | 0.97 ± 0.14 | 1.08 ± 0.10 | 1.12 ± 0.19 | 0.90 ± 0.08 |
| Lpl †,# | 0.12 ± 0.01 | 0.13 ± 0.01 | 0.10 ± 0.01 | 0.18 ± 0.02 | 0.15 ± 0.02 | 0.11 ± 0.01 | 0.10 ± 0.01 | 0.18 ± 0.03 |
| Pparg | 0.95 ± 0.18 | 0.91 ± 0.11 | 0.94 ± 0.17 | 0.91 ± 0.17 | 0.96 ± 0.22 | 0.78 ± 0.12 | 0.70 ± 0.10 | 0.75 ± 0.13 |
| Srebf1 | 0.82 ± 0.13 a | 0.64 ± 0.02 b | 0.89 ± 0.11 a | 0.52 ± 0.07 b | 0.86 ± 0.10 a | 0.65 ± 0.07 b | 0.79 ± 0.10 a | 0.40 ± 0.05 b |
| Gonadal Fat | ||||||||
| Fasn # | 3.23 ± 1.17 | 2.22 ± 0.52 | 2.36 ± 0.37 | 1.17 ± 0.06 | 0.85 ± 0.16 | 2.42 ± 0.46 | 3.08 ± 0.47 | 2.29 ± 0.59 |
| Lpl | 0.90 ± 0.05 | 0.94 ± 0.13 | 1.30 ± 0.33 | 0.80 ± 0.12 | 1.04 ± 0.30 | 0.88 ± 0.04 | 1.47 ± 0.27 | 1.07 ± 0.17 |
| Pparg | 0.84 ± 0.04 | 0.87 ± 0.08 | 0.95 ± 0.12 | 0.86 ± 0.03 | 0.68 ± 0.14 | 0.80 ± 0.12 | 0.81 ± 0.06 | 0.56 ± 0.09 |
| Srebf1 | 3.12 ± 0.65 | 3.05 ± 0.50 | 3.29 ± 0.51 | 2.21 ± 0.27 | 3.15 ± 0.64 a | 2.23 ± 0.18 b | 3.68 ± 0.63 a | 2.57 ± 0.45 b |
| Lep # | 0.90 ± 0.22 | 0.83 ± 0.08 | 1.01 ± 0.12 | 0.62 ± 0.12 | 0.38 ± 0.05 | 0.72 ± 0.12 | 1.32 ± 0.25 | 0.70 ± 0.14 |
| Retroperitoneal Fat | ||||||||
| Fasn | 5.19 ± 1.09 a | 2.35 ± 0.37 b | 3.01 ± 0.37 a | 2.19 ± 0.45 b | 1.86 ± 0.42 | 2.11 ± 0.47 | 2.13 ± 0.16 | 1.77 ± 0.57 |
| Lpl | 1.67 ± 0.16 a | 1.13 ± 0.17 b | 1.46 ± 0.11 a | 1.19 ± 0.18 b | 1.21 ± 0.14 a | 0.99 ± 0.11 b | 1.41 ± 0.13 a | 0.86 ± 0.04 b |
| Pparg # | 0.72 ± 0.03 | 0.95 ± 0.09 | 1.03 ± 0.20 | 1.15 ± 0.25 | 0.70 ± 0.07 | 1.01 ± 0.11 | 1.12 ± 0.22 | 0.71 ± 0.05 |
| Srebf1 | 2.71 ± 0.19 a | 1.89 ± 0.10 b | 2.47 ± 0.33 a | 1.74 ± 0.15 b | 2.93 ± 0.53 a | 2.27 ± 0.17 b | 2.61 ± 0.40 a | 1.77 ± 0.20 b |
| Lep # | 2.23 ± 0.39 a | 1.31 ± 0.10 b | 1.84 ± 0.15 a | 1.43 ± 0.20 b | 1.00 ± 0.08 | 1.03 ± 0.11 | 1.77 ± 0.13 | 0.95 ± 0.17 |
Values are means ± SEM and n = 6–9 per group. The effects of maternal dietary fatty acid ratio and maternal dietary fat content were analysed using a two-way ANOVA; all comparisons are made within sex group. Different superscripts (a, b) denote statistical different (p < 0.05). A significant interaction of maternal dietary fat content and maternal dietary fatty acid is denoted for † males (p < 0.05) and # females (p < 0.05). Fasn, fatty acid synthase; Lpl, lipoprotein lipase; Pparg, peroxisome proliferator-activated receptor gamma; Srebf1, sterol regulatory element-binding protein (variant 1c); Lep, leptin.