Table 4.
Offspring lipogenic gene expression in male and female offspring at 8 weeks of age.
| Experimental Group | Male | Female | ||||||
|---|---|---|---|---|---|---|---|---|
| High LA (18% Fat) |
High LA (36% Fat) |
Low LA (18% Fat) |
Low LA (36% Fat) |
High LA (18% Fat) |
High LA (36% Fat) |
Low LA (18% Fat) |
Low LA (36% Fat) |
|
| Liver | ||||||||
| Fasn † | 1.40 ± 0.14 | 2.23 ± 0.5 | 2.20 ± 0.34 | 1.58 ± 0.13 | 1.75 ± 0.16 | 2.45 ± 0.45 | 1.59 ± 0.29 | 2.43 ± 0.40 |
| Lpl | 0.12 ± 0.01 | 0.13 ± 0.02 | 0.12 ± 0.01 | 0.12 ± 0.01 | 0.13 ± 0.01 | 0.12 ± 0.02 | 0.10 ± 0.01 | 0.13 ± 0.01 |
| Pparg | 2.57 ± 0.53 | 2.25 ± 0.30 | 2.03 ± 0.22 | 1.71 ± 0.07 | 1.43 ±0.43 | 1.16 ± 0.29 | 0.85 ±0.15 | 0.94 ± 0.15 |
| Srebf1 | 1.80 ± 0.21 a | 1.63 ± 0.12 b | 2.12 ± 0.25 a | 1.56 ± 0.07 b | 1.39 ± 0.21 | 1.27 ± 0.14 | 1.10 ± 0.18 | 1.20 ± 0.17 |
| Gonadal Fat | ||||||||
| Fasn | 2.24 ± 0.64 | 2.70 ± 0.53 | 2.83 ± 0.51 | 4.03 ± 0.88 | 1.22 ± 0.35 a | 2.54 ± 0.23 b | 2.06 ± 0.59 a | 4.36 ± 1.10 b |
| Lpl | 2.91 ± 0.31 | 3.55 ± 0.52 | 3.50 ± 0.63 | 3.12 ± 0.36 | 2.00 ± 0.38 | 3.65 ± 0.78 | 3.06 ± 0.43 | 3.27 ± 0.53 |
| Pparg | 1.02 ± 0.08 | 0.90 ± 0.11 | 0.82 ± 0.06 | 1.00 ± 0.09 | 0.92 ± 0.07 | 0.94 ± 0.12 | 0.89 ± 0.14 | 1.02 ± 0.11 |
| Srebf1 | 3.18 ± 0.46 | 2.98 ± 0.17 | 3.65 ± 0.47 | 3.33 ± 0.56 | 2.48 ± 0.27 | 2.97 ± 0.33 | 3.14 ± 0.55 | 3.81 ± 0.69 |
| Lep | 4.79 ± 0.76 a | 4.12 ± 0.52 b | 5.82 ± 0.83 a | 3.58 ± 0.44 b | 2.34 ± 0.53 | 2.99 ± 0.47 | 4.44 ± 0.87 | 3.31 ± 0.58 |
| Retroperitoneal Fat | ||||||||
| Fasn | 1.58 ± 0.34 | 1.85 ± 0.31 | 1.75 ± 0.17 | 2.25 ± 0.37 | 0.64 ± 0.11 a | 2.24 ± 0.43 b | 1.11 ± 0.36 a | 1.53 ± 0.26 b |
| Lpl | 2.05 ± 0.45 | 2.01 ± 0.32 | 2.01 ± 0.19 | 2.41 ± 0.42 | 1.30 ± 0.21 | 2.08 ± 0.28 | 1.68 ± 0.21 | 1.54 ± 0.18 |
| Pparg | 0.94 ± 0.17 | 0.92 ± 0.04 | 0.88 ± 0.05 | 1.00 ± 0.06 | 0.92 ± 0.14 | 1.07 ± 0.09 | 1.06 ± 0.13 | 1.01 ± 0.04 |
| Srebf1 | 1.58 ± 0.30 | 1.48 ± 0.23 | 1.64 ± 0.10 | 1.79 ± 0.24 | 1.06 ± 0.07 | 1.81 ± 0.24 | 1.30 ± 0.12 | 1.29 ± 0.21 |
| Lep | 2.98 ± 0.68 | 2.62 ± 0.49 | 2.85 ± 0.33 | 2.47 ± 0.47 | 1.49 ± 0.33 | 1.95 ± 0.21 | 1.72 ± 0.24 | 1.49 ± 0.17 |
Values are means ± SEM and n = 6–9 per group. The effects of maternal dietary fatty acid ratio and maternal dietary fat content were analysed using a two-way ANOVA; all comparisons are made within sex group. Different superscripts (a, b) denote statistical different (p < 0.05). † Indicates a significant interaction of maternal dietary fat content and maternal dietary fatty acid in male offspring (p < 0.05). Fasn, fatty acid synthase; Lpl, lipoprotein lipase; Pparg, peroxisome proliferator-activated receptor gamma; Srebf1, sterol regulatory element-binding protein (variant 1c); Lep, leptin.