Figure 4.

3T3-L1 cells were left untreated or were pre-incubated with TAK-242 (TAK) 1 µM for 15 min, at 37 °C/5% CO₂. After pre-treatment, 3T3-L1 cells were treated or not with Lean Conditioned Medium (CM), Obese Conditioned Medium, or differentiation mix for 48 h. Thirty µg of proteins from cell lysates were subjected to Western blotting for C/EBPα (A) and PPARγ (B). Results are representative of three independent experiments. Data are expressed as means ± SEM. * Represents p < 0.05.