Figure 5.

3T3-L1 cells were left untreated or were pre-incubated with TAK-242 (TAK) 1 µM for 15 min, at 37 °C/5% CO₂. After pre-treatment, 3T3-L1 cells were treated or not with Lean Conditioned Medium (CM), Obese Conditioned Medium, or differentiation mix. After seven days, the images were registered in an optical microscope (A) or lipid were stained with Oil red O which was quantified in a plate cell reader (B). D-H. After six days the medium was removed, and cells were incubated with DMEM 1% FBS, for 24 h (to complete seven days). TNFα (C), IL-6 (D), IL-1β (E), leptin (F), and adiponectin (G) were measured in supernatants using a sandwich enzyme-linked immunosorbent assay kit. Results are representative of three independent experiments. B-H. Data are expressed as means ± SEM. * Represents p < 0.05, ** represents p < 0.01, **** represents p < 0.001.