Figure 4.

Pharmacological inhibition of IGF-1R decreases growth rate and bone formation in zebrafish larvae. Zebrafish larvae (n = 30) at 3 days post fertilization (dpf) were pretreated with 10 µM picropodophyllin (PPP) for 2 h prior to stimulation with 100 µg/mL FO or 4 mM β-glycerophosphate (GP). (A) Images of total body length were captured at 9 dpf (left) and the length is shown in the graph (right). (B) In a parallel experiment, the zebrafish larvae were stained with 0.05% calcein (top). Vertebrae number (bottom left) and relative density (bottom right) were calculated and are depicted in the graph. Significant differences among the groups were determined using one-way ANOVA followed by Bonferroni correction. All data are presented as mean ± SEM (* p < 0.05 and *** p < 0.001 vs. untreated zebrafish larvae). FO, fermented oyster (C. gigas) extract.