Table 1.

Zebrafish primers and PCR conditions used in this experiment.

Gene *	Primer Sequences (5′→3′)	Amplicon (bp)	Tm	Cycle No.
zccka	F: GAT GAA GAA CCT CGC AGC AG	154 bp	58 °C	27
	R: GGC CCA AAT CCA TCC ATC CC
zghr-1	F: TCA GTC CGA CTC AGA AAC CG	178 bp	58 °C	27
	R: TTC TGA AGC ACG GGA CCA TA
zgck	F: GTA GGT ACA GGC TGC AAT GC	224 bp	58 °C	27
	R: TCA CCC CTG TAC TTC CCA CC
zgh-1	F: GGT GGT ACA GGC TGC AAT GC	157 bp	58 °C	27
	R: CAA CTGTCT GCG TTC CTC AG
zghra	F: CAT TGT CAT TCC CCA GCA GC	214 bp	58 °C	27
	R: ATC TGC AGG ATC GTC GAT GT
Zigf-1	F: GAG TAC CCA CAC CCT CTC AC	213 bp	58 °C	27
	R: TGA AAG CAG CAT TCG TCC AC
ighfbp3	F: AGG ACA CCA TCA GAA CCC AG	182 bp	58 °C	27
	R: CGA CGA CAT GGG CCA TAT TC
zβactin	F: CGA GCG TGG CTA CAG CTT CA	155 bp	61 °C	27
	R: GAC CGT CAG GCA GCT CAT AG

bp: base pair, T_m: melting temperature, * zigf-1: insulin-like growth factor-1, zigfbp-3: insulin-like growth factor binding protein 3, zgh-1: growth hormone-1, zghr-1: growth hormone receptor-1, zghra: growth hormone receptor alpha, zgck: glucokinase, and zccka: cholecystokinin.