Figure 1.

Characterization of Niemann-Pick C1-Like 1 (NPC1L1)-dependent very low-density lipoprotein and low-density lipoprotein (VLDL/LDL) particles in Npc1l1^WT or Npc1l1^KO mice. (A) Cholesterol and (B) triglyceride concentrations in lipoprotein fractions, and (C) protein score, which reflects protein abundance and degree of confidence in protein identification, of apoM in VLDL/LDL fractions, collected from Npc1l1^WT or Npc1l1^KO male mice (initially 8–10 weeks old) fed a Western diet (WD) for the indicated periods in the absence (−) or presence (+) of ezetimibe (Eze). Representative lipoprotein profiles from two independent experiments with two mice in each group are shown. Bar graphs represent the mean ± S.D. Each dot on the bar graphs represents data from a single mouse (n = 3–4). * p < 0.05; ** p < 0.01, significant difference by Dunnett’s test compared with Npc1l1^WT Eze (−) mice.