Figure 2.

Phylogeny of genomes of C. botulinum Group I and C. sporogenes. Two major lineages (C. botulinum Group I (shown in black) and C. sporogenes (shown in red)) were identified. The phylogenetic tree was created by comparison of core single nucleotide polymorphisms identified using ParSNP program [70], Treegraph v2 [71], MEGA7 [72] and Figtree were used to annotate and visualize the phylogenetic tree. Accessory genes were identified using BLAST using reference sequences for the ha and orfX neurotoxin cluster configurations. The distance bar (0.05) represents the number of nucleotide substitutions per site for a given branch, based on the number of SNPs found in the core genome. Details of individual sequences and strains are given in Tables S1 and S2, where they appear in the same order as in this Figure. Newly sequenced strains are indicated (in black) as “new” in the right-hand column.