Figure 5.

EHV-1 spread between epithelium, PBMC, and EC. (A) ED cells were seeded into 3-µm pore size 24-well transwell inserts and incubated at 37 °C for 24 h. Subsequently, ED cells were infected with Ab4-wt or mutant viruses at an MOI of 0.1 for 1 h and treated with citrate buffer. Upon infection, transwell insert was inverted, and 1 × 10⁵ PBMC resuspended in medium containing virus-neutralizing antibodies were added. (B) Epithelium-to-PBMC virus transfer was assessed by counting the number of EGFP-positive cells after 24 h. (C) Subsequently, infected PBMC were used for flow chamber assay. The data represent means ± SD of three independent experiments. One-way ANOVA was done followed by correction for multiple comparisons; *—p < 0.05, **—p < 0.01. ns—not significant.