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PBMC cytokine and chemokine profile at 3, 6, and 24 hpi. Equine PBMC were infected with either Ab4-wt or mutant viruses at an MOI of 1. (A) At 3 hpi, (B) 6 hpi, and (C) 24 hpi, supernatants were collected, and cytokines and chemokines were quantified using a Milliplex® MAP equine cytokine/chemokine magnetic bead-based Multiplex kit with Luminex-based detection system. Mean concentrations of cytokines and chemokines are given in picogram (pg) per mL; (n = 2). hpi—hours post infection.
