Table 2.
Accuracy and sensitivity of LAMP, qPCR and PCR-RFLP assays for HTLV-1/2.
| Groups | LAMP | qPCR | PCR-RFLP | ||||
|---|---|---|---|---|---|---|---|
| n | Accuracy | Sensitivity | Accuracy | Sensitivity | Accuracy | Sensitivity | |
| Total 1 | 226 | 80.5 | 80.1 | 75.7 | 75.1 | 84.9 | 84.6 |
| (74.8–85.5) | (74.2–85.2) | (69.5–81.1) | (68.9–80.7) | (79.5–89.3) | (79.1–90.0) | ||
| HTLV-1 | 164 | 92.0 | 89.0 | 88.1 | 83.5 | 94.7 | 93.9 |
| (87.7–95.2) | (83.2–93.4) | (83.1–92.0) | (77.0–88.9) | (90.9–97.2) | (89.0–97.0) | ||
| HTLV-2 | 54 | 89.8 | 57.4 | 88.9 | 53.7 | 89.8 | 57.4 |
| (85.1–93.4) | (43.2–70.8) | (84.1–92.7) | (40.0–67.4) | (85.1–93.4) | (43.2–70.8) | ||
| HTLV-1/2 mono-infected | 144 | 88.9 | 88.9 | 79.9 | 79.9 | 93.1 | 93.1 |
| (82.6–93.5) | (82.6–93.5) | (72.4–86.1) | (72.4–86.1) | (87.6–96.6) | (87.6–96.6) | ||
| HIV-coinfected | 82 | 65.9 | 63.6 | 68.3 | 66.2 | 70.4 | 68.4 |
| (54.6–76.0) | (51.9–74.3) | (57.1–78.1) | (54.6–76.6) | (59.2–80.0) | (56.8–78.6) | ||
The overall accuracy and sensitivity of HTLV-1/2 LAMP, real-time quantitative PCR (qPCR), and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) assays are shown in percentage. 95% confidence intervals are presented inside parenthesis. 1 The total number of patients includes non-typed HTLV (n = 5) and indeterminate (n = 3) results.