Table 3.
Examples of RNAi-based gene silencing against Coronaviruses.
| Disease | Vector/Target | Effect | Ref |
|---|---|---|---|
| SARS | Hairpin cDNA/S1S2 siRNAs | Inhibition of replication in Vero E6 cells | [81] |
| S, nsP-12/13/16 siRNAs | 90% inhibition of replication in FRhK4 cells | [82] | |
| S, nsP-12 siRNAs | Suppression of SARS symptoms in macaques | [83] | |
| ACE2 shRNAs | Reduced infection in ACE2-silenced cells | [84] | |
| Ezrin siRNAs | Knock-down of ezrin | [85] | |
| MERS | ORF1ab siRNAs | Computational predictions for MERS control | [86] |
| ORF1ab miRNAs | Computational predictions for MERS control | [86] | |
| PDCoV | M/N shRNAs | Reduced titers and viral RNA in ST cells | [87] |
| PEDV | M shRNAs | Inhibition of viral RNA and replication | [88] |
| SADS | M shRNAs | Inhibition of viral RNA and replication | [89] |
| COVID-19 | ORF1b/3a/S,M/N siRNAs | Computational design of siRNAs | [90] |
ACE2, angiotensin converting enzyme-2; COVID-19, coronavirus disease; MERS-CoV, Middle East respiratory syndrome coronavirus; miRNAs, micro RNAs; PDCoV, porcine deltacoronavirus; PEDV, porcine epidemic diarrhea virus; SADS-CoV, swine acute diarrhea syndrome coronavirus; SARS-CoV, severe acute respiratory syndrome coronavirus: shRNAs, short hairpin RNAs; siRNAs, short interfering RNAs; ST, swine testicular.