Table 1.

Methods commonly used to purify virus and exosome are summarized based on their separation/ concentration efficiencies and scale range.

Methods	Separation	Concentration	Scale Range
Ultracentrifugation	+++	+++	5 to 250 mL
Normal flow filtration	+	++	0.5 to 1000 mL
Tangential flow filtration	+	++	100 to 5000 mL
Precipitation	-	+++	0.2 mL to >3 L
Size exclusion chromatography	+	-	0.5 mL to >3 L
Ion exchange chromatography	++	+	0.5 mL to >3 L
Affinity purification	++++	++	0.5 mL to >3 L