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Expression and solubility of the recombinant CHIKV-E2 protein produced in E. coli BL21 (DE3). (A) Recombinant CHIKV-E2 produced in the pellet (P, insoluble) and supernatant (S, soluble) fractions at different induction temperatures (18, 25 and 37 °C). (B) Solubility of the recombinant protein at different concentrations of urea (2, 4, 6 and 8 M).
