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Comparison of molecular mass and glycosylation analysis in CHIKV-E2 from HEK-293T. (A): Supernatant from HEK-293T cells and pellets from BL21 (DE3) cells were purified by affinity chromatography and evaluated in SDS-PAGE. (B): CHIKV-E2 produced in HEK-293T cells was treated with a PNGase F to remove all N-linked glycans and analyzed on western blot using anti-CHIKV mouse serum, showing that the higher molecular mass from the eukaryotic antigen is a result of protein glycosylation.
