Table 2.

PoAstV3 detection frequency and RT-qPCR mean Cq and Cq range by sample type over time.

Age (wks a)	Fecal Swabs		Oropharyngeal Swabs		Oral Fluids
	%	Mean Cq c	%	Mean Cq	%	Mean Cq
	(N b)	(Range)	(N)	(Range)	(N)	(Range)
3	95%	31.06	31%	37.66	NA d	NA d
	(61 of 64)	(16.78–38.88)	(20 of 64)	(32.34–39.27)
5	17%	35.68	2%	36.22	88%	32.92
	(11 of 64)	(27.07–39.59)	(1 of 64)	-	(7 of 8)	(28.31–36.23)
7	47%	33.63	5%	37.77	100%	31.95
	(28 of 60)	(24.07–39.55)	(3 of 60)	(36.90–38.55)	(8 of 8)	(28.63–34.49)
9	28%	34.85	2%	36.57	100%	31.73
	(15 of 53)	(26.50–38.77)	(1 of 53)	-	(8 of 8)	(27.18–34.25)
11	88%	34.29	24%	37.01	100%	32.20
	(44 of 50)	(27.38–38.54)	(12 of 50)	(34.43–39.94)	(8 of 8)	(30.52–32.50)
16	20%	36.37	2%	36.54	75%	36.14
	(10 of 49)	(30.33–39.57)	(1 of 49)	-	(6 of 8)	(33.36–38.50)
21	4%	37.37	0%	0	75%	35.73
	(2 of 47)	(36.29–38.45)	(0 of 47)	-	(6 of 8)	(33.13–38.28)
25	41%	34.57	4%	37.70	100%	34.23
	(19 of 46)	(30.27–37.85)	(2 of 46)	(37.57–37.84)	(8 of 8)	(33.22–35.48)

^a wks, Age in weeks of pigs sampled. ^b N, Number of samples tested by RT-qPCR. ^c Cq, Quantification cycle. ^d NA, Not available.