Table 3.

PoAstV3 detection frequency and RT-qPCR mean Cq and Cq range by environmental sample type over time.

Age (wks a)	Pens		Feeders		Hallway		Load-Out Chute
	%	Mean Cq c	%	Mean Cq	%	Mean Cq	%	Mean Cq
	(N b)	(Range)	(N)	(Range)	(N)		(N)
3	100%	31.01	100%	30.25	100%	31.07	100%	29.10
	(8 of 8)	(29.33–32.43)	(8 of 8)	(27.19–34.70)	(1 of 1)		(1 of 1)
5	63%	32.83	88%	32.17	100%	29.89	100%	30.28
	(5 of 8)	(30.12–34.77)	(7 of 8)	(27.63–37.83)	(1 of 1)		(1 of 1)
7	100%	32.13	100%	33.33	100%	29.16	100%	28.49
	(8 of 8)	(27.11–36.57)	(8 of 8)	(29.50–35.34)	(1 of 1)		(1 of 1)
9	88%	34.14	88%	33.23	100%	33.78	100%	35.37
	(7 of 8)	(29.79–39.40)	(7 of 8)	(27.64–36.46)	(1 of 1)		(1 of 1)
11	100%	36.31	88%	34.02	100%	32.90	100%	32.90
	(8 of 8)	(33.80–39.97)	(7 of 8)	(33.01–37.02)	(1 of 1)		(1 of 1)
16	88%	34.69	75%	35.35	100%	35.79	100%	33.41
	(7 of 8)	(32.81–39.42)	(6 of 8)	(33.64–37.59)	(1 of 1)		(1 of 1)
21	88%	36.49	25%	38.53	100%	32.66	100%	35.75
	(7 of 8)	(34.67–38.20)	(2 of 8)	(38.08–38.97)	(1 of 1)		(1 of 1)
25	75%	35.63	100%	35.99	100%	35.89	100%	34.00
	(6 of 8)	(34.30–37.77)	(8 of 8)	(33.03–39.74)	(1 of 1)		(1 of 1)

^a wks, Age in weeks of pigs sampled. ^b N, Number of samples tested by RT-qPCR. ^c Cq, Quantification cycle.