Table 4.
Aggregated PoAstV3 detection and RT-qPCR mean Cq and Cq range by environmental sample type over time.
| Sample Types | % | Mean Cq c | Mean gc d/mL of Samplee |
|---|---|---|---|
| (N b) | (Range) | (Range) | |
| Pens | 88% a | 33.98 a | 3.94 × 103 |
| (56 of 64) | (27.11–39.97) | (5.32 × 105–5.90 × 101) | |
| Feeders | 83% a | 33.75 a | 3.94 × 103 |
| (53 of 64) | (27.19–39.74) | (5.32 × 105–5.90 × 101) | |
| Hallway | 100% a | 32.63 a | 7.95 × 103 |
| (8 of 8) | (29.16–35.89) | (1.31 × 105–9.72 × 102) | |
| Load-out chute | 100% a | 32.18 a | 1.60 × 104 |
| (8 of 8) | (28.49–35.75) | (1.31 × 105–9.72 × 102) |
a No statistically significant difference (p < 0.05) between the detection rate or mean Cq of sample types. b N, Number of samples tested by RT-qPCR. c Cq, Quantification cycle. d gc, Genomic copies. e Formula for calculating gc/mL of sample 10((Cq−45.82)/−3.287).