Table 2.

Evaluation of assay performance in microtiter plates.

Plate Format	Sample	Ct Value		HBV Quantification (Copies/mL)		%CV 1		Z’ 2	Window 3
		DMSO	LMV	DMSO	LMV	DMSO	LMV
96	Intra	14.20 ± 0.08	23.41 ± 0.30	9.47 × 1010 ± 3.95 × 109	4.10 × 108 ± 9.76 × 107	2.56	0.91	0.88	230
	Extra	20.54 ± 0.17	28.06 ± 0.21	1.31 × 107 ± 1.77 × 106	4.71 × 104 ± 6.80 × 103	0.87	0.76	0.85	278
384	Intra	15.07 ± 0.35	21.33 ± 0.63	2.31 × 1010 ± 4.92 × 109	7.58 × 108 ± 1.88 × 108	2.34	2.96	0.53	30
	Extra	31.60 ± 0.80	44.80 ± 0.41	3.53 × 106 ± 1.33 × 105	2.80 × 103 ± 6.50 × 102	2.56	0.92	0.72	1260

¹ %CV (coefficient of variation). ² Z’ determined as described by Zhang et al., 1999 [9]. 0.5 ≤ Z’ < 1 indicates “high quality” of the assay. ³ Window (signal-to-background ratio).