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. 2020 Sep 4;12(9):982. doi: 10.3390/v12090982

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic representation of the IFAST device. It is formed by seven successive flow chambers containing three oil barriers. The magnet at the bottom of the device is moved from the input to the output wells at a rate of 1–2 mm per second. The paramagnetic particles (PMP) move with the magnet, carrying along attached cells and excluding unbound cells at the immiscible phase barrier. Isolated cells are dyed on-chip with a small molecule dye (Calcein AM) metabolized to a fluorophore to be read by a fluorometer [64].