Figure A3.

Conservation of Rac among members of Krylovirinae. (A) Genome comparison of phage phiKMV, LUZ19 and LKA1, scored according to tBLASTx comparison. Genes 25.1 and 28 are indicated with red arrows. (B) Phylogenetic tree (neighbor joining). (C) Affinity pull-down of P. aeruginosa rpoA::strep cells infected with phiKMV, LUZ19 and LKA1, followed by pull-down of the RNAP complex. After separation of these complexes on 12% SDS-PAGE gels, it is clear that the cleaved α RNAP phenotype can only be associated with infection of phages from the clusters with phiKMV and LUZ19. (D) Sequence alignment of a selection of Rac-like proteins of various clusters, with indication of the N-acetyl CoA binding pocket (QYV---------AR).