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Rac substrate profiling. (A) Immunoaffinity enrichment of the Rac substrates from P. aeruginosa cell lysate. The arrow indicates the additional acetylated protein in the presence of recombinant Rac and acetyl-CoA (lane 1), compared to the control sample (lane 2). (B) MS analysis reveals that HslU and ClpX are present in lane 1 but not in lane 2, with sequence coverage of 24% and 7%, respectively. The identified peptide sequences are underlined.
