Fig. 3.

Quantification of the Influence of Endog expression in the distribution of proliferating non-synchronized cells throughout the cell cycle phases. A) Skin fibroblast cultures were obtained from P3 Endog^+/+ (n = 3) and Endog^−/− (n = 6) mice and passaged two times. After 48 h in the plate, cells were detached, washed, fixed and stained with propidium iodide. The percentage of cells in each cell cycle phase was determined by flow cytometry. B) The same procedure was used for analyzing Rat-1 fibroblasts non transduced (NT) or transduced with scrambled (Scr) or Endog silencing constructs 1 and 2 (Endog shRNA) from 5 experiments; and C) for cultures of control HEK293 cells and HEK293 cell clones harboring a disruption of the ENDOG gene within exon 1 (4 experiments). Data depicted on stacked bar graphs are mean ± SEM. Statistical analysis was performed in A by the Student's t-test, and in C and D by one-way ANOVA followed by the Bonferroni test (ns: not significant; *. p < 0.05; **, p < 0.01; ***, p < 0.001).