Figure 3.

Epigenetic Profiling at Promoters in ID4-eGFP^Bright and ID4-eGFP^Dim Spermatogonia

(A–H) Scatterplots correlating H3K4me3 (x axis) and H3K27me3 (y axis) enrichments (log2RPKM) at promoters (TSS ±500 bp) in ID4-eGFP^Bright (A–C) and ID4-eGFP^Dim (E–G) spermatogonia. Color codes indicate gene expression levels (A, E), H2K27ac enrichment (B and F), and chromatin accessibility (C and G) at promoters. H3K27ac/H3K27me3 double ChIP-seq signal enrichment correlated with gene expression levels at promoters in ID4-eGFP^Bright and ID4-eGFP^Dim spermatogonia (D and H).

(I) Comparisons of histone modification, chromatin accessibility, and DNA methylation levels (RPKM, Reads Per Kilobase of sequence range per Million mapped reads) in the most differentially expressed gene sets in ID4-eGFP^Bright (red trace) and ID4-eGFP^Dim (blue trace) spermatogonia.