Figure 4:
Pso inhibits pro-survival signaling in ALDH+ and ALDH− cells. (A) Tumor tissues were processed for immunohistochemical staining for pAKT (pro-survival).Total of 300–400 cells were counted and out of that pAKT positive cells were counted and are represented in the graph as percent. (B) ALDH+(Control), ALDH+(Pso), ALDH− (Control) and ALDH− (Pso) tumor were utilized to prepare whole cell lysates. Western blot analysis was done for AKT, phosphorylated-AKT (pAKT), p65 and Bcl-2. was used as a control for equal loading. The graph represents the densitometric analysis (Fold) for each protein normalized to β-actin.