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. 2020 Sep 6;10(9):1589. doi: 10.3390/ani10091589

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative photomicrographs of RAC1 protein immunolocalization (in brown) in the prehierarchical follicle of ovarian. Panel (A,B), oocytes and GCs were strongly stained within the different size of prehierarchical follicles; Panel (C,D), a larger developing prehierarchical follicles, which contains two or three layers of GCs; Panel (E), a larger prehierarchical follicles and more layers of GCs and thecal cells developing in this stage; Panel (F), RAC1 negative controls, which were treated with pre-immune serum and no specific staining was observed. Oocyte (OC), granulosa cell (GC), theca cell (TC), stroma (ST) and nucleus (N) are indicated. Scale bar = 200 μm (A,B,F); 100 μm (C,D,E).