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. 2020 Sep 6;10(9):1589. doi: 10.3390/ani10091589

Figure 4.

Figure 4

Effects of silencing RAC1 on granulosa cell proliferation. The effects of silencing RAC1 on the proliferation of GCs were studied by EdU cell proliferation assay. (A) The GCs were transfected with different treatment groups. SR group: exact siRNA RAC1; NC group: scrambled siRNA; BC group: absence of siRNA. All nuclei of cells showed a blue fluorescence suggestive of Hoechst33342 staining. The red fluorescence showed in EdU-labeled cells suggesting the newly produced DNA (original magnification ×20) (Figure S1, original magnification ×40). (B) The proliferation percentage of GC post cell transfected with the RAC1 specific siRNA. Data represent means ± SEM (n = 10). Bars with superscripts suggest the statistically noteworthy difference in comparison to the control groups (* p < 0.05).