Figure 6.

Effects of overexpressing RAC1 on granulosa cell proliferation. The effects EdU cell proliferation assay was used to assess the effects of overexpressing RAC1 on GC proliferation. (A) The GCs were transfected with different treatment groups. OE group: pYr-adshuttle-4-RAC1 vector; NC group: pYr-adshuttle-4 empty vector; BC group: absence of expression vector. Blue fluorescence was seen in all cell nuclei since Hoechst33342 staining, cells labeled with red fluorescence suggesting their newly produced DNA (original magnification ×20) (Figure S2, original magnification ×40). (B) The percentage of GC proliferation after cells were transfected with pYr-adshuttle-4-RAC1 vector. Data represent means ± SEM (n = 10). Bars with superscripts suggest the statistically noteworthy difference in comparison to the control groups (* p < 0.05).