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. 2020 Sep 6;10(9):1589. doi: 10.3390/ani10091589

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Rac1 stimulates mRNA expression of FSHR, CCND2, PCNA, StAR and CYP11A1 genes. (A) Different treatment groups were transfected into GCs from the prehierarchical follicles.SR group: exact siRNA RAC1; NC group: scrambled siRNA; BC group: absence of siRNA. Transfection knocked down RAC1 on FSHR, CCND2, PCNA, StAR and CYP11A1 genes mRNA expression. (B) Different treatment groups were transfected into GCs from the prehierarchical follicles. OE group: pYr-adshuttle-4-RAC1 vector; NC group: pYr-adshuttle-4 empty vector; BC group: absence of expression vector. Transfection overexpressed RAC1 on FSHR, CCND2, PCNA, StAR and CYP11A1 mRNA expression. Data represent means ± SEM (n = 10). Bars with superscripts suggest the statistically noteworthy difference in comparison to the control groups (* p < 0.05).