Table 2.
The composition of the complete dietetic commercial mixtures of feed used in the experiment.
| Type of Feed | |||
|---|---|---|---|
| Name | Broiler Starter Prestige | Broiler Grower Prestige | Broiler Finisher Prestige |
| Composition | wheat, corn, post-extraction soya meal *, hemoglobin (from swine blood), rapeseed cake, soybean oil *, calcium carbonate, palm oil derived fatty acids, monocalcium phosphate, sodium chloride | wheat, corn, post-extraction soya meal *, hemoglobin (from swine blood), rapeseed cake, post-extraction sunflower meal, palm oil derived fatty acids, soybean oil *, calcium carbonate, swine fat, monocalcium phosphate, sodium chloride | wheat, corn, post-extraction soya meal * rapeseed cake, soybean oil *, calcium carbonate, monocalcium phosphate, sodium chloride |
| Component amount in 1 kg of feed | |||
| Total proteins (Kjeldahl method) |
225 g | 205 g | 187.5 g |
| Oil and crude fats | 48 g | 55 g | 75 g |
| Fiber | 27 g | 30 g | 33 g |
| Lysine | 13.6 g | 12.9 g | 12.2 g |
| Methionine | 6 g | 5.8 g | 5.6 g |
| Calcium | 8 g | 6.2 g | 4.9 g |
| Phosphorus | 6 g | 5.2 g | 4.6 g |
| Sodium | 1.5 g | 1.5 g | 1.4 g |
| Ash | 55 g | 47 g | 40 g |
| Dietetic additives in 1 kg of feed | |||
| Vitamin A (3a672a) | 10,000 IU | ||
| Vitamin D3 (E671) | 5000 IU | ||
| Vitamin E (dl-α-tocopherol) | 75 mg | ||
| Fe (iron sulphate, E1) | 40 mg | ||
| J (potassium iodide, 3b201) | 1.25 mg | ||
| Cu (copper sulphate, E4) | 16 mg | ||
| Mn (manganese oxide, E5) | 120 mg | ||
| Zn (zinc oxide, E6) | 100 mg | ||
| Se (sodium selenite, E8) | 0.3 mg | ||
| Zootechnics additives in 1 kg of feed | |||
| 6-phytase (EC 3.1.3.26) 500 FTU/g, 4a19 | 1000 FTU | 1000 FTU | - |
| 6-phytase (EC 3.1.26) 2500 OTU/g, 4a16 | - | - | 250 OTU/g |
| Endo-1,4-β-xylanase (EC 3.2.1.8), 12,500 VU/mL, 4a22 | 1250 VU | 1250 VU | - |
| Endo-1,4-β-xylanase (EC 3.2.1.8), 30,000 EPU/g, 4a1617 | - | - | 1500 EPU |
| Endo-1,3 (4)-β-gluconate (EC 3.2.1.6), 8600 VU/mL, 4a22 | 860 VU | 860 VU | - |
| Serine protease (EC 3.4.21), 75,000 PROT/g 4a13 | 15000 PROT | - | - |
EPU—one endo-1,4-β-xylanase unit is the amount of enzyme which releases 0.0083 μmol of reducing sugars (xylose equivalent) per minute from oat spelt xylan at pH 4.7 and 50 °C; FTU—one 6-phytase unit is the amount of enzyme which releases 1 µmol of inorganic phosphate from sodium phytate in one minute at 37 °C and pH 5.5; PROT—one protease unit is the amount of enzyme which releases 1 µmol of p-nitroaniline from 1 mM substrate (Suc-Ala-AlaPro-Phe-pNA) in one minute at pH 9.0 and temperature 37 °C; VU—one endo-1,3(4)-β-gluconate unit is the amount of enzyme which hydrolyzes substrate (β-glucan of barley and arabinoxylan of wheat, respectively), while reducing viscosity of the solution, so that there is a change in relative fluidity of 1 (dimensionless unit) in one minute at 30 °C and pH 5.5; *—produced from GMO soybeans (MON 40-3-2).