Table 1.

PCR primers used in this study.

Target Microorganism	Target Gene	Primer	Sequence (5′–3′)	Amplicon Size (bp)	Annealing Temperature (°C)	References
E. coli	malB	malBF	TCGCCACACGCTGACGCTGACCA	585	55	[16]
		malBR	TTACATGACCTCGGTTTAGTTCACAGA
E. faecalis	ddl1	ddl1F	TGTTGTATGGCGGCAGAAGT	941	54	[17]
		ddl1R	TCAGGTGTTTGTGCCCAAGT
E. faecium	ddl2	ddl2F	ATGGGACCCAAGTGGACAGA	550	54	[17]
		ddl2R	ATTTCGCGCGCTTCAATTCC
S. aureus	nuc	NucF	GCGATTGATGGTGATACGGTT	279	55	[18]
		NucR	AGCCAAGCCTTGACGAACTAAAGC
Streptococcus	16S-23S rRNA	SU-F2	AGCCGCCTAAGGTGGGAT	220–230	60	[19]
		SU-R	ATGGAGCCTAGCGGGATC