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. 2020 Aug 21;10(9):1467. doi: 10.3390/ani10091467

Figure 3.

Figure 3

Protein tyrosine phosphorylation in stallion spermatozoa after 180 min of incubation for capacitation. (A) Micrograph of fluorescence microscopy of stallion spermatozoa after 180 min of capacitation and immune-labelled for protein tyrosine phosphorylation (PTP) (green). Nuclei were labelled with Hoechst 33342 (blue), bar = 10 µm. Pattern I: spermatozoa showing PTP at the midpiece. Pattern II: Spermatozoa showing PTP along the whole flagellum. (B,C) Percentage of spermatozoa showing pattern I (B) and pattern II (C) staining at time 0 and after 180 min of incubation in Whitten’s medium (WHI), synthetic human tubal fluid (HTF), or HTF supplemented with 750 µM of penicillamine (HTF + PEN). a, b, c Different letters indicate significant differences (p < 0.05, n = 6, 12 determinations).