Figure 5.

Protein tyrosine phosphorylation in the thermotaxis assay. (A,B) Percentage of spermatozoa showing staining patterns I or II for tyrosine phosphorylation in the unselected sample, in the spermatozoa migrating across constant temperatures (35 or 38 °C), or across a temperature gradient (from 35 to 38 °C, thermotaxis). (C) Representative micrograph of immunofluorescence for tyrosine phosphorylation (green) in stallion spermatozoa migrating by thermotaxis. Nuclei were labelled with Hoechst 33342 (blue). Bar = 10 µm. (D) Percentage of spermatozoa showing staining patterns I and II for protein tyrosine phosphorylation in the unselected sample or in those spermatozoa that did not migrate during the thermotaxis assay in the conditions described above. *** p = 0.001, ^{a, b, c} different letters indicate significant differences (p < 0.05, n = 6).