Table 5.
Associative effects † of the combinations between plant extracts rich in T and S added to the HF or HC diets.
| Diet | Extract | Gas 24 h (mL/g DM) | Gas 48 h (mL/g DM) | CH4 24 h (mL/L gas) | CH4 48 h (mL/L gas) | IVDMD 48 h (mg/g) | IVOMD 48 h (mg/g) | Total VFA 48 h (mmol/L) | Ammonia 48 h (mmol/L) |
|---|---|---|---|---|---|---|---|---|---|
| HF | T25S75 | 4.4 | 4.1 | −4.9 ** | −3.2 * | −5.6 | −1.7 | 7.8 | −8.2 *** |
| T50S50 | 3.9 | 3.0 | −2.0 | −1.9 | −4.6 | −6.9 | −21.1 * | −16.3 *** | |
| T75S25 | 3.7 * | 0.9 | 1.3 | 0.1 | −2.2 | 3.0 | 1.3 | −19.6 *** | |
| HC | T25S75 | −4.6 | −2.1 | −0.6 | −1.8 | 2.2 | 2.3 | 8.1 | −1.5 |
| T50S50 | 2.4 | 3.0 ** | 1.0 | −0.1 | −3.0 | −1.8 | 12.2 | −6.8 ** | |
| T75S25 | 2.1 | 2.0 | 1.3 | 0.4 | 0.9 | 0.2 | 11.7 | −13.5 ** |
* p < 0.05; ** p < 0.01; *** p < 0.001; † Difference between observed values (obtained by measurements) and expected values (arithmetic means of the values obtained with incubations with exclusively plant extracts rich in tannins or saponins) in percent of the expected values; HF: 700 mg/g Napier grass and 300 mg/g concentrate; HC: 300 mg/g Napier grass and 700 mg/g concentrate; CH4: methane; DM: dry matter; IVDMD: in vitro dry matter degradability; IVOMD: in vitro organic matter degradability; and VFA: volatile fatty acids. Indices describe percentages of the respective extracts.