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. 2020 Sep 16;10(9):1658. doi: 10.3390/ani10091658

Figure 1.

Figure 1

(A) The schematic diagram of the strategy for deleting the yqeI gene in AE81: (B) confirmation of the mutant strain AE81ΔyqeI and the complemented strain AE81ΔyqeI-pCmΔyqeI. M: 5000 DNA marker; Lane 1: PCR product (1309 bp) amplified from AE81 with primers yqeI-out; Lane 2: PCR product (577 bp) amplified from AE81ΔyqeI with primers yqeI-out; Lane 3: negative control with primers yqeI-out; Lane 4: PCR product (870 bp) amplified from AE81 with primers C-yqeI; Lane 5: PCR product (870 bp) amplified from AE81ΔyqeI-pCmΔyqeI with primers C-yqeI; Lane 6: negative control with primers C-yqeI; (C) the growth curves of AE81, AE81ΔyqeI and AE81ΔyqeI-pCmΔyqeI in lysogeny broth.